| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169859 |
| Chromosome: | chromosome 2 |
| Location: | 2394630 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091200 | (1 of 1) 1.13.11.5 - Homogentisate 1,2-dioxygenase / Homogentisicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAGGTCTAAGGGAGGCGGCAACCGGAA |
| Internal bar code: | TGGTTCAAGACGGAGGGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 91.6239 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCCAAACGAGACCCTT |
| Suggested primer 2: | GGGCATAGCTGGTCAGGATA |