Insertion junction: LMJ.RY0402.169868_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.169868
Chromosome:chromosome 6
Location:172167
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g250300 DHC16,DHC1b,FLA24 (1 of 1) K10414 - dynein heavy chain 2, cytosolic (DYNC2H, DNCH2); Cytoplasmic Dynein 1b Heavy Chain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGACTGTGGAGGTGGACAAGAACGCGG
Internal bar code:GCGAACAGCAGTCGGTCCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:97.2973
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCTGGCCAAGGACAAAA
Suggested primer 2:GATCTCGGCCTCAATCTCTG