Insertion junction: LMJ.RY0402.169877_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.169877
Chromosome:chromosome 9
Location:3190855
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g387356 (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGGTGCCGTACCATGAGTACTTTGAGC
Internal bar code:ATATGCGTCGTCCACTTGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:99.5547
LEAP-Seq n confirming:7602
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAAAGCTTGGTGTCAGAG
Suggested primer 2:ATGAGCAGCTGTACCCCATC