| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169930 |
| Chromosome: | chromosome 1 |
| Location: | 1008017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005750 | (1 of 11) IPR002350 - Kazal domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATTGCTCAAGCATTAGCATGGCCAGAC |
| Internal bar code: | TAGGAGGGACGAGAGCAAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.3154 |
| LEAP-Seq n confirming: | 1886 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCAGGTTGCTTACTCC |
| Suggested primer 2: | ACGGTAAATGCAAATGCACA |