Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.169931 |
Chromosome: | chromosome_14 |
Location: | 3615026 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g631200 | DII6,IC97,FAP94 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTTTGGGTGGTCGCGTGGTCCGCGGGTTG |
Internal bar code: | CGGAGGTGGAAAGTTGAATAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 645 |
LEAP-Seq percent confirming: | 93.7879 |
LEAP-Seq n confirming: | 619 |
LEAP-Seq n nonconfirming: | 41 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCTTCCCTTGTACTCCCC |
Suggested primer 2: | TCAGCGTTTCAGACTTGTGG |