| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169939 |
| Chromosome: | chromosome 10 |
| Location: | 5775055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461250 | NSF1 | (1 of 1) K06027 - vesicle-fusing ATPase (NSF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTCGCCATTCCTGGCGGTTCAAACTCG |
| Internal bar code: | ACACAGGGTGAATCGAACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.7577 |
| LEAP-Seq n confirming: | 1647 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGACGAGATGGTCAAGGT |
| Suggested primer 2: | CGCTAGCAAGTAGGGTGAGG |