| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169972 |
| Chromosome: | chromosome 1 |
| Location: | 5565355 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039550 | PLAP1,PLP1 | Plastid lipid associated protein 1; (1 of 230) IPR017986 - WD40-repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGTTGCAGGCTCCAGCACTCTGCTGC |
| Internal bar code: | AGTTTACTGAAATGAGATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.6564 |
| LEAP-Seq n confirming: | 4060 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTACTCATCCCCGAAC |
| Suggested primer 2: | ATCCTTGATGCGATACTGGC |