| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.170039 |
| Chromosome: | chromosome 13 |
| Location: | 2081567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577450 | GPA3 | (1 of 1) 1.1.5.3 - Glycerol-3-phosphate dehydrogenase / sn-glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate acyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGCAGTCAAACTTACAGCAAATAAAC |
| Internal bar code: | GTCTCGGGGCGGGTGTTTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.8496 |
| LEAP-Seq n confirming: | 1328 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGCTCCTTTGAAGACAG |
| Suggested primer 2: | CGCCATAGTGTGATGGTTTG |