| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170066 |
| Chromosome: | chromosome 16 |
| Location: | 648675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691800 | FLVB1,FLVB | (1 of 2) 1.5.3.22 - Coenzyme F420H(2) oxidase; Thylakoid flavodiiron protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGCCTGGAGCTCCTTCTGCAGCGCCT |
| Internal bar code: | GGAGGGATTCAACGTTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 99.786 |
| LEAP-Seq n confirming: | 1399 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATCTCCTCCATCTCCAC |
| Suggested primer 2: | TTGTATAGCAGGTGGGGGAG |