Insertion junction: LMJ.RY0402.170078_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.170078
Chromosome:chromosome 12
Location:1095738
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g490750 (1 of 239) IPR016024 - Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGCATCTCCAGGGGCAGTCCGGCGGCC
Internal bar code:CTTTGTTTATAGGACCGCGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:196
LEAP-Seq percent confirming:72.7273
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGCTACTCCCATTGACAC
Suggested primer 2:GCTGTCTCGGAGTGTCCTTC