Insertion junction: LMJ.RY0402.170094_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170094
Chromosome:chromosome 14
Location:3751784
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g632250 (1 of 2) IPR006502//IPR011333//IPR011705 - Protein of unknown function PDDEXK-like // POZ domain // BTB/Kelch-associated CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTATCTGCCAGGCTGGGATATAAATGA
Internal bar code:TGGAAACTCGGGCTAGGAAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:704
LEAP-Seq percent confirming:99.3976
LEAP-Seq n confirming:495
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTACTGCGTACGACATCGC
Suggested primer 2:GCCATTGTCGTTGATGTGTC