| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170104 |
| Chromosome: | chromosome 1 |
| Location: | 4406762 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029850 | STK19,STPK19 | (1 of 1) PTHR24356//PTHR24356:SF183//PTHR24356:SF212 - SERINE/THREONINE-PROTEIN KINASE // AGC (CAMP-DEPENDENT, CGMP-DEPENDENT AND PROTEIN KINASE C) KINASE FAMILY PROTEIN // AGC (CAMP-DEPENDENT, CGMP-DEPENDENT AND PROTEIN KINASE C) KINASE FAMILY PROTEIN; Serine/threonine protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCTCTCGAACTGCGCATTAGATGCAGG |
| Internal bar code: | AGAGATCACGTAAAGCTTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.5981 |
| LEAP-Seq n confirming: | 3717 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACGCCTCAAAGTCCTCT |
| Suggested primer 2: | GGCCAAACAGAAGAGAGTGC |