| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170110 |
| Chromosome: | chromosome 3 |
| Location: | 4027595 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172200 | (1 of 12) IPR001680//IPR015943//IPR017986 - WD40 repeat // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGGGCGCTTTAGGTTGCTTACAGAGCG |
| Internal bar code: | GGATTCCCACAAGTGCACGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.8463 |
| LEAP-Seq n confirming: | 1299 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCTTGCACTCCTTGCTC |
| Suggested primer 2: | AGACGTAGATGTTCCCGGTG |