| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170130 |
| Chromosome: | chromosome 2 |
| Location: | 6493297 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116350 | (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGCAGGCGGTGTGTCAACTGGCCAGT |
| Internal bar code: | GGGAGCAACCCTTATCCGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.7611 |
| LEAP-Seq n confirming: | 1670 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCGTTTAACGTTCCAT |
| Suggested primer 2: | ACGTCCAGTCTATGTTGGGC |