| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170134 |
| Chromosome: | chromosome 7 |
| Location: | 3486804 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGACAACTGCTCGATGACGCGTCCGCG |
| Internal bar code: | CTGTCTTTGCTCGAGTGGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1193 |
| LEAP-Seq percent confirming: | 99.0234 |
| LEAP-Seq n confirming: | 1521 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAACCTCGCTCAGAACTT |
| Suggested primer 2: | TTTGGAAAGGTGCTGAGCTT |