| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170141 |
| Chromosome: | chromosome 9 |
| Location: | 1441311 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398400 | TRP5 | Transient receptor potential ion channel protein; (1 of 46) PF00520 - Ion transport protein (Ion_trans) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGCGCCAGAATGCCGGCGATGGGAGAC |
| Internal bar code: | GCTCGTTGGAGGGTATCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 98.7152 |
| LEAP-Seq n confirming: | 461 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTAACCGCTCAGAGTTG |
| Suggested primer 2: | CTTGTTCAGCTTCTCCGACC |