| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170161 |
| Chromosome: | chromosome 9 |
| Location: | 3052187 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387300 | (1 of 1) PTHR12707:SF0 - PININ | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACGCCTTGCGCCCAAGCCCTGACAC |
| Internal bar code: | GACGCAACGAAACTTGCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.6871 |
| LEAP-Seq n confirming: | 2549 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCCGTGGAGTTGGACAT |
| Suggested primer 2: | CGTGATGTTTGAAGTCCCCT |