Insertion junction: LMJ.RY0402.170163_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.170163
Chromosome:chromosome 2
Location:2058360
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g088750 ATR2 (1 of 1) K08874 - transformation/transcription domain-associated protein (TRRAP); ATM/ATR-related phosphatidylinositol 3-kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCATGAATGTAACCGCCTTACCTTGAG
Internal bar code:GGTCGATAGCTCGATGCGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:99.3927
LEAP-Seq n confirming:1964
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCACAACTGTGCTCCTCGTC
Suggested primer 2:AATGGAGATTCGTCACGGTC