| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.170175 |
| Chromosome: | chromosome 8 |
| Location: | 1759381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366150 | ATG16 | Autophagy related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGCAACAGGTGCGTACCGGTACAGGG |
| Internal bar code: | TCGTATTGACTACCGACATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.6984 |
| LEAP-Seq n confirming: | 6612 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTTCGTCAGTGTCTTGG |
| Suggested primer 2: | CTGTCATACACGCATGGGAC |