| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170183 |
| Chromosome: | chromosome 17 |
| Location: | 666873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700650 | GFY2 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACGCCCCCTGGCCCCTTCTGGGCCGT |
| Internal bar code: | TATGTCCCGAAGAGATGCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.2876 |
| LEAP-Seq n confirming: | 2230 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTCATGTGCAAGCTGCC |
| Suggested primer 2: | ATAAGGCTGAGCTGGGGATT |