| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170184 |
| Chromosome: | chromosome 2 |
| Location: | 441373 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076250 | EFG1 | Chloroplast elongation factor G | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGCGCCGGCCTTGGACTTGGCCACGA |
| Internal bar code: | CGACTCAGGGGAAACGAATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 1866 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACGGAGTTGACCTTGG |
| Suggested primer 2: | TGTGGAAAAGAAGAATGGGC |