| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170185 |
| Chromosome: | chromosome 10 |
| Location: | 3906059 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447900 | (1 of 1) PF12239 - Protein of unknown function (DUF3605) (DUF3605) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCAGGCATTGGCTGGGTCGTGACGTG |
| Internal bar code: | GCAAGTCCCGTTCTGGCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.7343 |
| LEAP-Seq n confirming: | 1126 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAAACAGCAGGCGAGTG |
| Suggested primer 2: | ACGTGGAGGGCTACAAACAC |