Insertion junction: LMJ.RY0402.170234_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170234
Chromosome:chromosome 3
Location:109568
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGCTGACTGTGTGGCGTGTGTGTATCG
Internal bar code:TAGGGAAATTCTGGGGGAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:241
LEAP-Seq percent confirming:2.56024
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:647
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TATCGAACTTGTGAGCGTGC
Suggested primer 2:TTTGTCTATCGGCAGCTGTG