Insertion junction: LMJ.RY0402.170234_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170234
Chromosome:chromosome 3
Location:109619
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGTTCGCTGACTACACTACGATCCTGCC
Internal bar code:CGGGGAAATGCTCTAACTTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:99.8414
LEAP-Seq n confirming:1888
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGTCTATCGGCAGCTGTG
Suggested primer 2:TATCGAACTTGTGAGCGTGC