| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170235 |
| Chromosome: | chromosome 1 |
| Location: | 321884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001800 | MLCK1,FAP403 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; Flagellar Associated Protein 403 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCATGCCGCCGCCATGCATGGGCCTCAA |
| Internal bar code: | GTATTACCAAGGCTTTGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.4048 |
| LEAP-Seq n confirming: | 668 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACATTCGCTGTCCTAC |
| Suggested primer 2: | AGCGAAGTCTGTGTCGTCCT |