| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.170290 |
| Chromosome: | chromosome 12 |
| Location: | 3724915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514800 | (1 of 2) K06287 - septum formation protein (maf) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGTATACATTCTCCCTCTGTCGTGCC |
| Internal bar code: | GCACGGGGAGGAACGCTGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 87.6133 |
| LEAP-Seq n confirming: | 290 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAAGCTGAGGGTACGGG |
| Suggested primer 2: | ACACCCATTGCTTGTGTTCA |