Insertion junction: LMJ.RY0402.170307_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170307
Chromosome:chromosome 9
Location:6134177
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g405300 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTCGCAGCTAGCGGTGCCAAATGACGG
Internal bar code:CCGGACGATTTGGACCGATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:260
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAACTTGGGTTGCCTTCA
Suggested primer 2:GAGGAAGAGGAGTTTGTGCG