Insertion junction: LMJ.RY0402.170311_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170311
Chromosome:chromosome 9
Location:7529229
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g414150 GT90F42,GT90-42 GT90 family protein 42; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTTGGGGGGTCGGTCCTCACGTCCCTGG
Internal bar code:ACCCATCATGAAGGGCGCTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:737
LEAP-Seq percent confirming:95.6155
LEAP-Seq n confirming:567
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGTACCCACACCTCGAT
Suggested primer 2:TCTGCTTCGTTTGTGTTTGC