Insertion junction: LMJ.RY0402.170329_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.170329
Chromosome:chromosome 11
Location:2557881
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476150 Glutamine cyclotransferase; (1 of 1) 2.3.2.5 - Glutaminyl-peptide cyclotransferase / Glutaminyl-tRNA cyclotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACCACATCATCATCACACCTCACAACA
Internal bar code:GTTTGAAAGTTTCGATATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1474
LEAP-Seq percent confirming:99.1035
LEAP-Seq n confirming:1216
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCTGACCACTACGGGACC
Suggested primer 2:TCAAGTTCAGTGCCGACAAG