| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170365 |
| Chromosome: | chromosome_9 |
| Location: | 6535358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g407801 | AKC1 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGCGATGTTGCGGTAGCCGCGGGAGTCC |
| Internal bar code: | AGTCCACCGGTCTACACTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTGATCAAGGTGCTC |
| Suggested primer 2: | CACACACACACACAAACCGA |