Insertion junction: LMJ.RY0402.170376_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170376
Chromosome:chromosome 2
Location:6960055
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g120301 SELH,SELENOH,SELH1 (1 of 133) IPR012336 - Thioredoxin-like fold; Selenoprotein H intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGCTGCCGCCGCTGCTGGTGCTGCTAC
Internal bar code:CCGCGCGGCTCATCGTCGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1016
LEAP-Seq percent confirming:87.0011
LEAP-Seq n confirming:3815
LEAP-Seq n nonconfirming:570
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCACAACAACTTAAACGC
Suggested primer 2:AGGTGGCGACAAATGATAGG