| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170392 |
| Chromosome: | chromosome 1 |
| Location: | 6783552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048900 | GTF1 | Global Transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCAGTAGTCCTGATGTGTGCAAGCGGC |
| Internal bar code: | GGGCAAGTAAGTATTCTGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.6071 |
| LEAP-Seq n confirming: | 11663 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCCAGAAACATGCACTT |
| Suggested primer 2: | AGGCAAAGTACGGCTTGCTA |