| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170400 |
| Chromosome: | chromosome 2 |
| Location: | 6582591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117150 | (1 of 1) PTHR23077:SF10 - PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCAGCGAGAGCGGCAAGCTGGTGCG |
| Internal bar code: | GCTGCTGTTCGCGCCGAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.4943 |
| LEAP-Seq n confirming: | 3345 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCCCAATCTACATTCC |
| Suggested primer 2: | GTGTGTGTGCGTGTGTGTGT |