| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170417 |
| Chromosome: | chromosome 14 |
| Location: | 1649057 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619050 | (1 of 1) PTHR31465:SF1 - PROTEIN RTA1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGAACTCGACCGTGCGGAAGATGTTC |
| Internal bar code: | TTCATCATTCAGTGTGGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 98.3793 |
| LEAP-Seq n confirming: | 5463 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCACTTGCCGAGGAAGG |
| Suggested primer 2: | CTCATCATCACGCCAGTCAT |