| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170442 |
| Chromosome: | chromosome 16 |
| Location: | 1967655 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656433 | TNP41,CSB59 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTAATTGCATACTGCATCGGCAGCTCG |
| Internal bar code: | ACGCGGCGTGCCGAACGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 97.5713 |
| LEAP-Seq n confirming: | 924 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCTTGGAGGTCTTGTA |
| Suggested primer 2: | CTCAGACTGTGAAGGGGCTC |