| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.170445 |
| Chromosome: | chromosome 12 |
| Location: | 2215162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508250 | (1 of 1) PTHR22975:SF9 - F6D8.33-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCGAACCCTGCATCCAGGAACCCAGC |
| Internal bar code: | GGGTCCATAGTCGGTCAGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.7322 |
| LEAP-Seq n confirming: | 9309 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTGGGTGATGTTCGAC |
| Suggested primer 2: | ATGTAAGCATTTTCAGGCGG |