| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170456 |
| Chromosome: | chromosome 16 |
| Location: | 728620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691150 | (1 of 11) PF13360 - PQQ-like domain (PQQ_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGGAGGTGCCGGGCACTACACCGTGC |
| Internal bar code: | GCAGGTGTTACTTTTGTCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 96.3938 |
| LEAP-Seq n confirming: | 4945 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTTCGAAGACGAGAAC |
| Suggested primer 2: | CCTTCTCCAGAACGAGATGC |