| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170459 |
| Chromosome: | chromosome 2 |
| Location: | 7017497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147700 | (1 of 1) PTHR11271//PTHR11271:SF33 - CHLOROHYDROLASE FAMILY PROTEINS // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTCTATGAAAGGCAAAGGGTGGAGGGG |
| Internal bar code: | GATATCACGCTTTCTCAGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 99.2991 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTACCGTGACTTGCCCAC |
| Suggested primer 2: | CGCATCTACAGAAGGTGCAA |