Insertion junction: LMJ.RY0402.170487_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.170487
Chromosome:chromosome 12
Location:1206682
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g489600 (1 of 3) IPR003029//IPR012340//IPR022967 - S1 domain // Nucleic acid-binding, OB-fold // RNA-binding domain, S1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCACGCCACATCCCGCAACCAACAAGTA
Internal bar code:AACTCATCACATCTTCAATATC

Confirmation - LEAP-Seq

LEAP-Seq distance:870
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:550
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAAAGAGGAGTCCGCTCA
Suggested primer 2:TCGATGCGACAGACACTAGG