| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170496 |
| Chromosome: | chromosome_14 |
| Location: | 2393000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g624400 | FAP99 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCACATCCTACATACTTCAAACGCAAAATA |
| Internal bar code: | CGCCCGCGGTACCGTCGCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 98.8896 |
| LEAP-Seq n confirming: | 3295 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTATTTGTGAACGGCGACC |
| Suggested primer 2: | CAATCTACTCTGCGCCCTTC |