| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170499 |
| Chromosome: | chromosome 3 |
| Location: | 5645284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187350 | TUD1,UNI3 | (1 of 1) K10390 - tubulin delta (TUBD); Delta tubulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTTGCCGCTGCCGCTCTGCATGACCAG |
| Internal bar code: | AGTTTTCATCCGAAAGAGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.8819 |
| LEAP-Seq n confirming: | 1691 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGAACTAGCCCAGCCT |
| Suggested primer 2: | CCAGTTGAGTTGTCCAGGGT |