| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.170509 |
| Chromosome: | chromosome 10 |
| Location: | 4264803 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450550 | PAO3,PAO1 | Pheophorbide a oxygenase; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATACGTGGCTGCGGACGTGCCTACCT |
| Internal bar code: | GCTTGAGCCCACGCTGACATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.4273 |
| LEAP-Seq n confirming: | 868 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGGCCTCCAGCTGACAC |
| Suggested primer 2: | TCTTGATAGACAGGGTGGGC |