| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.170509 |
| Chromosome: | chromosome 10 |
| Location: | 4960276 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCAAAGGCGCCGCCGCCGGAAGCCTG |
| Internal bar code: | TGGATAGGGCATTTTCCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 5.99369 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 596 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTGCGCAAAGACAGTG |
| Suggested primer 2: | CATACATAACGGCATCGTCG |