| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170530 |
| Chromosome: | chromosome 2 |
| Location: | 3239566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095076 | ROC39,MFT10 | Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTGTATATGATGGCCGCAACCATGCCC |
| Internal bar code: | TGCGCTCCACAGCACGGAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 95.6305 |
| LEAP-Seq n confirming: | 1532 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGCGTTTCGTTCTTAAC |
| Suggested primer 2: | ATGGGCACTCCTTCGTACAC |