Insertion junction: LMJ.RY0402.170555_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170555
Chromosome:chromosome 7
Location:6335415
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g357250 protein of unknown function; (1 of 1) PTHR24188//PTHR24188:SF41 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCACGGCGCCCTCCGCTCCCCTACCAG
Internal bar code:AACCTGACGGGGAACCGCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:578
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:730
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCAACATCACAACCAGC
Suggested primer 2:TGGGGGTCCCATCATACTTA