| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170640 |
| Chromosome: | chromosome 11 |
| Location: | 1801577 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467500 | (1 of 1) PF05664 - Plant family of unknown function (DUF810) (DUF810) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTCGGCAGCTGGCTACCGTATTGGCGT |
| Internal bar code: | ATGGGACGAATTATGGGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.2502 |
| LEAP-Seq n confirming: | 2912 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTGGAGAGGAGGTGAAG |
| Suggested primer 2: | CCTTGCCCTCTCCTTATTCC |