| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.170658 |
| Chromosome: | chromosome 12 |
| Location: | 2662550 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504250 | (1 of 3) 2.7.11.25//2.7.12.1 - Mitogen-activated protein kinase kinase kinase / MLTK // Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACGCTGCAGGATGCTACCCCGCGGG |
| Internal bar code: | GGCGTAGAAGAGGGCCTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 89.9025 |
| LEAP-Seq n confirming: | 2858 |
| LEAP-Seq n nonconfirming: | 321 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTACTAGTGCGTGTTCG |
| Suggested primer 2: | ATACCACGGGTCTTACTGCG |