Insertion junction: LMJ.RY0402.170661_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.170661
Chromosome:chromosome 6
Location:8196045
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g305450 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTGGCGCGAAATCCTCGTCCAGGTCCT
Internal bar code:CCGAGAACGCCCAACGGTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:33
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACCAACGGTACACGCA
Suggested primer 2:CTAAAGCACATGTCTCCGCA