Insertion junction: LMJ.RY0402.170679_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170679
Chromosome:chromosome 13
Location:3742950
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTGTCGTACGGCTTGCAGTTGCTGTAC
Internal bar code:ACTACCTTCGTGGTGGCCAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:93.954
LEAP-Seq n confirming:777
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCTCAGAGCTAACCACC
Suggested primer 2:GACGGTGGAGTTCAGCTACC