Insertion junction: LMJ.RY0402.170699_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.170699
Chromosome:chromosome 3
Location:4125516
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g173300 (1 of 6) IPR007577//IPR029044 - Glycosyltransferase, DXD sugar-binding motif // Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTAAACCAAACCAAGCGAAAACGAGGGAG
Internal bar code:TGCGAGAGAAAGCGGCGAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:984
LEAP-Seq percent confirming:93.4913
LEAP-Seq n confirming:10888
LEAP-Seq n nonconfirming:758
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:GATCCCCACCTAACAGGGAT
Suggested primer 2:CAGAGAGGAGACGGACAAGC